BmrD

From SubtiWiki
Revision as of 08:46, 19 September 2014 by Jstuelk (talk | contribs) (This gene is a member of the following regulons)
Jump to: navigation, search
  • Description: multidrug ABC transporter (ATP-binding protein), also involved in the signalling pathway to activate KinA at the onset of sporulation

Gene name bmrD
Synonyms yheH
Essential no
Product multidrug ABC transporter (ATP-binding protein)
Function multiple antibiotic resistance
Gene expression levels in SubtiExpress: bmrD
Interactions involving this protein in SubtInteract: BmrD
MW, pI 76 kDa, 7.082
Gene length, protein length 2019 bp, 673 aa
Immediate neighbours bmrC, yheG
Sequences Protein DNA DNA_with_flanks
Genetic context
YheH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BmrD expression.png















Categories containing this gene/protein

ABC transporters, resistance against toxins/ antibiotics, membrane proteins, sporulation proteins

This gene is a member of the following regulons

AbrB regulon, BmrB regulon

The gene

Basic information

  • Locus tag: BSU09720

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: has both a membrane-spanning and an ATP-binding domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in response to antibiotics that target protein synthesis (BmrB) PubMed
    • bmrC-bmrD expression only occurs during the late-exponential and stationary growth stages, irrespective of the timing of the antibiotic challenge (AbrB) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ewoud Reilman, Ruben A T Mars, Jan Maarten van Dijl, Emma L Denham
The multidrug ABC transporter BmrC/BmrD of Bacillus subtilis is regulated via a ribosome-mediated transcriptional attenuation mechanism.
Nucleic Acids Res: 2014, 42(18);11393-407
[PubMed:25217586] [WorldCat.org] [DOI] (I p)

Carmen Galián, Florence Manon, Manuela Dezi, Cristina Torres, Christine Ebel, Daniel Lévy, Jean-Michel Jault
Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization.
PLoS One: 2011, 6(5);e19677
[PubMed:21602923] [WorldCat.org] [DOI] (I p)

Cristina Torres, Carmen Galián, Christoph Freiberg, Jean-Raphaël Fantino, Jean-Michel Jault
The YheI/YheH heterodimer from Bacillus subtilis is a multidrug ABC transporter.
Biochim Biophys Acta: 2009, 1788(3);615-22
[PubMed:19167342] [WorldCat.org] [DOI] (P p)

Sanae Fukushima, Mika Yoshimura, Taku Chibazakura, Tsutomu Sato, Hirofumi Yoshikawa
The putative ABC transporter YheH/YheI is involved in the signalling pathway that activates KinA during sporulation initiation.
FEMS Microbiol Lett: 2006, 256(1);90-7
[PubMed:16487324] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)