DisA
- Description: DNA integrity scanning protein,diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spore's genome is free of damage
Gene name | disA |
Synonyms | yacK |
Essential | no |
Product | DNA integrity scanning protein, has diadenylate cyclase activity |
Function | control of sporulation initiation |
Gene expression levels in SubtiExpress: disA | |
Interactions involving this protein in SubtInteract: DisA | |
Metabolic function and regulation of this protein in SubtiPathways: disA | |
MW, pI | 40 kDa, 5.569 |
Gene length, protein length | 1080 bp, 360 aa |
Immediate neighbours | radA, yacL |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell division, metabolism of signalling nucleotides, sporulation/ other, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins
This gene is a member of the following regulons
CtsR regulon, SigB regulon, SigM regulon
The gene
Basic information
- Locus tag: BSU00880
Phenotypes of a mutant
Database entries
- BsubCyc: BSU00880
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family: disA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains a DAC domain for the synthesis of c-di-AMP PubMed
- Modification:
Database entries
- BsubCyc: BSU00880
- UniProt: P37573
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- GP987 (disA::tet), available in Jörg Stülke's lab
- BKG2 (radA-disA::spc), available in Jörg Stülke's lab
- 1A939 ( disA::tet), PubMed, available at BGSC
- Expression vector:
- IPTG inducible expression of His-disA in E. coli: pGP2563 (in pET19b), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086]
[WorldCat.org]
[DOI]
(I e)
Original publications
Silvia S Campos, Juan R Ibarra-Rodriguez, Rocío C Barajas-Ornelas, Fernando H Ramírez-Guadiana, Armando Obregón-Herrera, Peter Setlow, Mario Pedraza-Reyes
Interaction of apurinic/apyrimidinic endonucleases Nfo and ExoA with the DNA integrity scanning protein DisA in the processing of oxidative DNA damage during Bacillus subtilis spore outgrowth.
J Bacteriol: 2014, 196(3);568-78
[PubMed:24244006]
[WorldCat.org]
[DOI]
(I p)
Lei Zhang, Zheng-Guo He
Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.
J Biol Chem: 2013, 288(31);22426-36
[PubMed:23760274]
[WorldCat.org]
[DOI]
(I p)
Cao Zheng, Jieping Wang, Yunchao Luo, Yang Fu, Jianmei Su, Jin He
Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis.
Enzyme Microb Technol: 2013, 52(6-7);319-24
[PubMed:23608499]
[WorldCat.org]
[DOI]
(I p)
Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352]
[WorldCat.org]
[DOI]
(I p)
Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650]
[WorldCat.org]
[DOI]
(I p)
Gregor Witte, Sophia Hartung, Katharina Büttner, Karl-Peter Hopfner
Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.
Mol Cell: 2008, 30(2);167-78
[PubMed:18439896]
[WorldCat.org]
[DOI]
(I p)
Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969]
[WorldCat.org]
[DOI]
(P p)
Michal Bejerano-Sagie, Yaara Oppenheimer-Shaanan, Idit Berlatzky, Alex Rouvinski, Mor Meyerovich, Sigal Ben-Yehuda
A checkpoint protein that scans the chromosome for damage at the start of sporulation in Bacillus subtilis.
Cell: 2006, 125(4);679-90
[PubMed:16713562]
[WorldCat.org]
[DOI]
(P p)
Erik Boye
DisA, a busy bee that monitors chromosome integrity.
Cell: 2006, 125(4);641-3
[PubMed:16713555]
[WorldCat.org]
[DOI]
(P p)
Sigal Ben-Yehuda, David Z Rudner, Richard Losick
RacA, a bacterial protein that anchors chromosomes to the cell poles.
Science: 2003, 299(5606);532-6
[PubMed:12493822]
[WorldCat.org]
[DOI]
(I p)
A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224]
[WorldCat.org]
[DOI]
(P p)
I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115]
[WorldCat.org]
[DOI]
(P p)
E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870]
[WorldCat.org]
[DOI]
(P p)