ctsR

ctsR
168

transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)

Locus
BSU_00830
Molecular weight
17.60 kDa
Isoelectric point
9.26
Protein length
154 aaSequenceBlast
Gene length
465 bpSequenceBlast
Function
regulation of protein degradation
Product
transcription repressor
Essential
no
Synonyms
ctsR, yacG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4463 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
101,449  101,913
Phenotypes of a mutant
reduced expression of trxB, due to reduced Spx activity PubMed
The protein
Protein family
CtsR family (single member, according to UniProt)
Structure
3H0D (PDB) (complex with a 26bp DNA duplex, from Geobacillus stearothermophilus) PubMed
6FH4 (PDB) (C-terminal domain bound to phosphoarginine) PubMed
P37568 (AlphaFold)
Modification
phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by ClpC-ClpP) PubMed
phosphorylation of a tyrosine residue by McsB PubMed
recently, it was reported that CtsR is phosphorylated by McsB on Arg-62 rather than on a tyrosine residue PubMed
in addition, Arg-15 was reported to be a phosphorylation site PubMed
Effectors of protein activity
CtsR is inactivated by heat, heat sensing requires Gly-64 PubMed
non-phosphorylated McsB targets CtsR for degradation PubMed
regulated proteolysis by ClpP/ClpC PubMed
Expression and Regulation
Operons
Genes
ctsR-mcsA-mcsB-clpC-radA-disA
Description
PubMed
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed PubMed, in sigB regulon
SigF: sigma factor, PubMed, in sigF regulon
Open in new tab

ctsR→disA

2025-03-17 18:53:30

Bzhu

158

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
GP3202(ctsR::ermC), available in Jörg Stülke's lab
MGNA-B928 (yacG::erm), available at the NBRP B. subtilis, Japan
ctsR::aphA3 availbale in Ulf Gerth's lab
BKE00830 (''ctsR::erm'', available in the BGSC and in Jörg Stülke's lab) PubMed
BKE00830 (ctsR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC,  downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
BKK00830 (ctsR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC,  downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP3798 (spc, based on pGP1331), available in Jörg Stülke's lab
Antibody
available in Ulf Gerth's lab
References
Reviews
Loading
Original Publications
Loading

908DB17A39D518E84977250C55825E77FA02E391

Page visits: 8861

Time of last update: 2025-03-28 12:39:18

Author of last update: Jstuelk