Dat

• Description: D-alanine aminotransferase
  
  

Gene name	dat
Synonyms	yheM
Essential	no
Product	D-alanine aminotransferase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: dat
Metabolic function and regulation of this protein in SubtiPathways: dat
MW, pI	31 kDa, 4.871
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yheN, nhaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

The gene

Basic information

• Locus tag: BSU09670

Database entries

• BsubCyc: BSU09670

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09670

• Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

• UniProt: O07597

• KEGG entry: [2]

• E.C. number: 2.6.1.21

Expression and regulation

• Operon:

• Expression browser: dat PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1262 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 794 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

P P Taylor, I G Fotheringham
Nucleotide sequence of the Bacillus licheniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species.
Biochim Biophys Acta: 1997, 1350(1);38-40
[PubMed:9003455] [WorldCat.org] [DOI] (P p)