BacD

• Description: alanine-anticapsin ligase
  
  

Gene name	bacD
Synonyms	ywfE, ipa-83d
Essential	no
Product	alanine-anticapsin ligase
Function	biosynthesis of the antibiotic bacilysin
Gene expression levels in SubtiExpress: bacD
MW, pI	52 kDa, 4.679
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	bacE, bacC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ScoC regulon

The gene

Basic information

• Locus tag: BSU37710

Database entries

• BsubCyc: BSU37710

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ligation of anticapsin to L-Ala to form bacilysin, ultimate step in bacilysin biosynthesis PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37710

• Structure: 3VMM PubMed

• UniProt: P39641

• KEGG entry: [2]

• E.C. number: 6.3.2.28

Expression and regulation

• Operon: bacA-bacB-bacC-bacD-bacE-bacF PubMed

• Expression browser: bacD PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takeo Tsuda, Mana Asami, Yoshiaki Koguchi, Shuichi Kojima
Single mutation alters the substrate specificity of L-amino acid ligase.
Biochemistry: 2014, 53(16);2650-60
[PubMed:24702628] [WorldCat.org] [DOI] (I p)

Jared B Parker, Christopher T Walsh
Action and timing of BacC and BacD in the late stages of biosynthesis of the dipeptide antibiotic bacilysin.
Biochemistry: 2013, 52(5);889-901
[PubMed:23317005] [WorldCat.org] [DOI] (I p)

Yasuhito Shomura, Emi Hinokuchi, Hajime Ikeda, Akihiro Senoo, Yuichi Takahashi, Jun-ichi Saito, Hirofumi Komori, Naoki Shibata, Yoshiyuki Yonetani, Yoshiki Higuchi
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.
Protein Sci: 2012, 21(5);707-16
[PubMed:22407814] [WorldCat.org] [DOI] (I p)

Takeo Tsuda, Tomomi Suzuki, Shuichi Kojima
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2012, 68(Pt 2);203-6
[PubMed:22298000] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839] [WorldCat.org] [DOI] (I p)

Türkan Ebru Köroğlu, Ismail Oğülür, Seval Mutlu, Ayten Yazgan-Karataş, Gülay Ozcengiz
Global regulatory systems operating in Bacilysin biosynthesis in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2011, 20(3);144-55
[PubMed:21709425] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Kuniki Kino, Yoichi Kotanaka, Toshinobu Arai, Makoto Yagasaki
A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin.
Biosci Biotechnol Biochem: 2009, 73(4);901-7
[PubMed:19352016] [WorldCat.org] [DOI] (I p)

Kazuhiko Tabata, Hajime Ikeda, Shin-Ichi Hashimoto
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
J Bacteriol: 2005, 187(15);5195-202
[PubMed:16030213] [WorldCat.org] [DOI] (P p)

Ayten Yazgan Karataş, Seçil Cetin, Gülay Ozcengiz
The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis.
Biochim Biophys Acta: 2003, 1626(1-3);51-6
[PubMed:12697329] [WorldCat.org] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)