IolG

• Description: inositol 2-dehydrogenase
  
  

Gene name	iolG
Synonyms	idh, iol
Essential	no
Product	inositol 2-dehydrogenase
Function	myo-inositol catabolism
Gene expression levels in SubtiExpress: iolG
Metabolic function and regulation of this protein in SubtiPathways: iolG
MW, pI	38 kDa, 4.865
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	iolH, iolF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

• Locus tag: BSU39700

Database entries

• BsubCyc: BSU39700

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Myo-inositol + NAD⁺ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH (according to Swiss-Prot)

• Protein family: gfo/idh/mocA family (according to Swiss-Prot)

• Paralogous protein(s): YfiI, NtdC, YrbE, YteT, YulF, IolW, IolX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: NAD⁺ PubMed

• Effectors of protein activity:

• Interactions:
  • the protein forms a tetramer PubMed

• Localization:

Database entries

• BsubCyc: BSU39700

• Structure: 3MZ0 PubMed

• UniProt: P26935

• KEGG entry: [3]

• E.C. number: 1.1.1.18

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ PubMed

• Expression browser: iolG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan