BacF

• Description: aminotransferase involved in bacilysin synthesis
  
  

Gene name	bacF
Synonyms	ywfG, ipa-85d
Essential	no
Product	aminotransferase
Function	biosynthesis of the antibiotic bacilysin
Gene expression levels in SubtiExpress: bacF
MW, pI	44 kDa, 5.347
Gene length, protein length	1197 bp, 399 aa
Immediate neighbours	bacG, bacE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

AbrB regulon, CodY regulon, ScoC regulon

The gene

Basic information

• Locus tag: BSU37690

Database entries

• BsubCyc: BSU37690

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: YwfG catalyzes transamination of tetrahydro-4-hydroxyphenylpyruvate (with L-Phe as amino donor), to form tetrahydrotyrosine PubMed

• Protein family: class-I pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37690

• Structure:

• UniProt: P39643

• KEGG entry: [2]

• E.C. number: 2.6.1.1

Expression and regulation

• Operon: bacA-bacB-bacC-bacD-bacE-bacF PubMed

• Expression browser: bacF PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839] [WorldCat.org] [DOI] (I p)

Türkan Ebru Köroğlu, Ismail Oğülür, Seval Mutlu, Ayten Yazgan-Karataş, Gülay Ozcengiz
Global regulatory systems operating in Bacilysin biosynthesis in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2011, 20(3);144-55
[PubMed:21709425] [WorldCat.org] [DOI] (I p)

Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Ayten Yazgan Karataş, Seçil Cetin, Gülay Ozcengiz
The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis.
Biochim Biophys Acta: 2003, 1626(1-3);51-6
[PubMed:12697329] [WorldCat.org] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)