GerBC

• Description: lipoprotein, nutrient receptor, germination response to the combination of glucose, fructose, and KCl
  
  

Gene name	gerBC
Synonyms
Essential	no
Product	nutrient receptor
Function	germination
Gene expression levels in SubtiExpress: gerBC
Interactions involving this protein in SubtInteract: GerBC
MW, pI	42 kDa, 9.06
Gene length, protein length	1122 bp, 374 aa
Immediate neighbours	gerBB, ywtG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU35820

Database entries

• BsubCyc: BSU35820

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gerABKC lipoprotein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerBA-GerBB-GerBC (tentative receptor complex) PubMed
  • GerBC is part of the germinosome in the spore inner membrane PubMed

• Localization:
  • outer surface of the inner spore membrane PubMed

Database entries

• BsubCyc: BSU35820

• Structure: 3N54 PubMed

• UniProt: P39571

• KEGG entry: [3]

• E.C. number:

Additional information

• 700 molecules are present per spore PubMed

Expression and regulation

• Operon: gerBA-gerBB-gerBC PubMed

• Expression browser: gerBC PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:
  • 700 molecules are present per spore PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kerry-Ann V Stewart, Peter Setlow
Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis.
J Bacteriol: 2013, 195(16);3575-82
[PubMed:23749970] [WorldCat.org] [DOI] (I p)

Jing-qiao Zhang, Keren K Griffiths, Ann Cowan, Peter Setlow, Ji Yu
Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination.
J Bacteriol: 2013, 195(8);1735-40
[PubMed:23396907] [WorldCat.org] [DOI] (I p)

Krzysztof Hinc, Krzysztofa Nagórska, Adam Iwanicki, Grzegorz Wegrzyn, Simone J Séror, Michal Obuchowski
Expression of genes coding for GerA and GerK spore germination receptors is dependent on the protein phosphatase PrpE.
J Bacteriol: 2006, 188(12);4373-83
[PubMed:16740944] [WorldCat.org] [DOI] (P p)

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Kerry-Ann V Stewart, Xuan Yi, Sonali Ghosh, Peter Setlow
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.
J Bacteriol: 2012, 194(12);3156-64
[PubMed:22493018] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Yunfeng Li, Parvathimadhavi Catta, Kerry-Ann V Stewart, Matthew Dufner, Peter Setlow, Bing Hao
Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor.
J Bacteriol: 2011, 193(16);4143-52
[PubMed:21685283] [WorldCat.org] [DOI] (I p)

Yunfeng Li, Barbara Setlow, Peter Setlow, Bing Hao
Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor.
J Mol Biol: 2010, 402(1);8-16
[PubMed:20654628] [WorldCat.org] [DOI] (I p)

Swaroopa Atluri, Katerina Ragkousi, Donna E Cortezzo, Peter Setlow
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor.
J Bacteriol: 2006, 188(1);28-36
[PubMed:16352818] [WorldCat.org] [DOI] (P p)

Takao Igarashi, Peter Setlow
Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores.
J Bacteriol: 2005, 187(7);2513-8
[PubMed:15774895] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Rosa-Martha Cabrera-Martinez, Federico Tovar-Rojo, Venkata Ramana Vepachedu, Peter Setlow
Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis.
J Bacteriol: 2003, 185(8);2457-64
[PubMed:12670969] [WorldCat.org] [DOI] (P p)

B M Corfe, A Moir, D Popham, P Setlow
Analysis of the expression and regulation of the gerB spore germination operon of Bacillus subtilis 168.
Microbiology (Reading): 1994, 140 ( Pt 11);3079-83
[PubMed:7812448] [WorldCat.org] [DOI] (P p)

B M Corfe, R L Sammons, D A Smith, C Mauël
The gerB region of the Bacillus subtilis 168 chromosome encodes a homologue of the gerA spore germination operon.
Microbiology (Reading): 1994, 140 ( Pt 3);471-8
[PubMed:8012571] [WorldCat.org] [DOI] (P p)