YcnK

• Description: copper-responsive transcription repressor of the ycnK-ycnJ-ycnI operon
  
  

Gene name	ycnK
Synonyms
Essential	no
Product	transcription repressor, DeoR family
Function	regulation of copper uptake
Gene expression levels in SubtiExpress: ycnK
MW, pI	21 kDa, 7.371
Gene length, protein length	570 bp, 190 aa
Immediate neighbours	ycnJ, ycnL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons

AbrB regulon, YcnK regulon

The YcnK regulon:

• ycnK-ycnJ-ycnI

The gene

Basic information

• Locus tag: BSU03960

Phenotypes of a mutant

• elevated expression of ycnJ under conditions of copper excess PubMed

Database entries

• BsubCyc: BSU03960

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed

• Protein family: DeoR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): copper acts as corepressor PubMed

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed

• Localization:

Database entries

• BsubCyc: BSU03960

• Structure:

• UniProt: P94433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycnK-ycnJ-ycnI PubMed

• Expression browser: ycnK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by copper limitation (YcnK) PubMed
  • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • YcnK: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Original publications

Kazutake Hirooka, Takayosh Edahiro, Kosuke Kimura, Yasutaro Fujita
Direct and indirect regulation of the ycnKJI operon involved in copper uptake through two transcriptional repressors, YcnK and CsoR, in Bacillus subtilis.
J Bacteriol: 2012, 194(20);5675-87
[PubMed:22904286] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Shashi Chillappagari, Marcus Miethke, Hein Trip, Oscar P Kuipers, Mohamed A Marahiel
Copper acquisition is mediated by YcnJ and regulated by YcnK and CsoR in Bacillus subtilis.
J Bacteriol: 2009, 191(7);2362-70
[PubMed:19168619] [WorldCat.org] [DOI] (I p)

Mélanie A Hamon, Nicola R Stanley, Robert A Britton, Alan D Grossman, Beth A Lazazzera
Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.
Mol Microbiol: 2004, 52(3);847-60
[PubMed:15101989] [WorldCat.org] [DOI] (P p)