SpoIVFA

• Description: inhibitor of SpoIVFB metalloprotease
  
  

Gene name	spoIVFA
Synonyms	bofB, spoVL
Essential	no
Product	inhibitor of SpoIVFB metalloprotease
Function	control of SigK activation
Gene expression levels in SubtiExpress: spoIVFA
Interactions involving this protein in SubtInteract: SpoIVFA
MW, pI	29 kDa, 7.292
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	spoIVFB, minD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU27980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PDZ protease PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • the ectodomain of SpoIVFA is cleaved off by SpoIVB (first cleavage) PubMed
  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SpoIVFB-SpoIVFA, to facilitate the recruitment of BofA to SpoIVFB PubMed
  • BofA-SpoIVFA PubMed
  • SpoIVB-SpoIVFA PubMed
  • CtpB-SpoIVFA PubMed

• Localization:
  • mother cell membrane PubMed
  • integral membrane protein PubMed

Database entries

• Structure:

• UniProt: P26936

• KEGG entry: [3]

• E.C. number:

Additional information

• • the ectodomain of SpoIVFA is cleaved off by SpoIVB (first cleavage) PubMed
  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) {{PubMed|24243021}

Expression and regulation

• Operon: spoIVFA-spoIVFB PubMed

• Expression browser: spoIVFA PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:
  • the ectodomain of SpoIVFA is cleaved off by SpoIVB (first cleavage) PubMed
  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: