MutL

• Description: DNA mismatch repair
  
  

Gene name	mutL
Synonyms
Essential	no
Product	DNA mismatch repair
Function	DNA repair
Gene expression levels in SubtiExpress: mutL
Interactions involving this protein in SubtInteract: MutL
MW, pI	70 kDa, 5.583
Gene length, protein length	1881 bp, 627 aa
Immediate neighbours	mutS, ymzD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17050

Phenotypes of a mutant

• 60-fold increased mutation rate PubMed
• a rnhB mutS-mutL mutant is not viable PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DNA mismatch repair mutL/hexB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MutL (C-terminal domain)-DnaN PubMed
  • DnaE-MutL PubMed
  • MutL-MutS PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed

Database entries

• Structure: 3KDK (C-terminal domain) PubMed

• UniProt: P49850

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutL PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between mutL and ymzD PubMed

Biological materials

• Mutant:
  • GP1190 (del mutS-mutL::aphA3) PubMed, available in Jörg Stülke's lab
  • 1A833 ( mutL::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Justin S Lenhart, Monica C Pillon, Alba Guarné, Lyle A Simmons
Trapping and visualizing intermediate steps in the mismatch repair pathway in vivo.
Mol Microbiol: 2013, 90(4);680-98
[PubMed:23998896] [WorldCat.org] [DOI] (I p)

Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084] [WorldCat.org] [DOI] (I p)

Nicholas J Bolz, Justin S Lenhart, Steven C Weindorf, Lyle A Simmons
Residues in the N-terminal domain of MutL required for mismatch repair in Bacillus subtilis.
J Bacteriol: 2012, 194(19);5361-7
[PubMed:22843852] [WorldCat.org] [DOI] (I p)

Katrin Gunka, Stefan Tholen, Jan Gerwig, Christina Herzberg, Jörg Stülke, Fabian M Commichau
A high-frequency mutation in Bacillus subtilis: requirements for the decryptification of the gudB glutamate dehydrogenase gene.
J Bacteriol: 2012, 194(5);1036-44
[PubMed:22178973] [WorldCat.org] [DOI] (I p)

Andrew D Klocko, Jeremy W Schroeder, Brian W Walsh, Justin S Lenhart, Margery L Evans, Lyle A Simmons
Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.
Mol Microbiol: 2011, 82(3);648-63
[PubMed:21958350] [WorldCat.org] [DOI] (I p)

Monica C Pillon, Jeffrey H Miller, Alba Guarné
The endonuclease domain of MutL interacts with the β sliding clamp.
DNA Repair (Amst): 2011, 10(1);87-93
[PubMed:21050827] [WorldCat.org] [DOI] (I p)

Monica C Pillon, Jessica J Lorenowicz, Michael Uckelmann, Andrew D Klocko, Ryan R Mitchell, Yu Seon Chung, Paul Modrich, Graham C Walker, Lyle A Simmons, Peter Friedhoff, Alba Guarné
Structure of the endonuclease domain of MutL: unlicensed to cut.
Mol Cell: 2010, 39(1);145-51
[PubMed:20603082] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mario Pedraza-Reyes, Ronald E Yasbin
Contribution of the mismatch DNA repair system to the generation of stationary-phase-induced mutants of Bacillus subtilis.
J Bacteriol: 2004, 186(19);6485-91
[PubMed:15375129] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)