SpoIIIE

• Description: ATP-dependent DNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
  
  

Gene name	spoIIIE
Synonyms
Essential	no
Product	ATP-dependent DNA translocase required for chromosome partitioning through the septum into the forespore
Function	chromosome partition during sporulation
Gene expression levels in SubtiExpress: spoIIIE
MW, pI	86 kDa, 6.757
Gene length, protein length	2361 bp, 787 aa
Immediate neighbours	ylzJ, ymfC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU16800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • transports the forespore chromosome across the sporulation septum PubMed
  • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed

• Protein family:

• Paralogous protein(s): SftA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • assembles into ∼45 nm complexes PubMed

• Localization:
  • transmembrane domain mediates dynamic localization to active division sites PubMed
  • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

Database entries

• Structure:

• UniProt: P21458

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIE PubMed

• Expression browser: spoIIIE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: