YxaL

• Description: similar to Ser/Thr kinase, increases the processivity of the PcrA helicase
  
  

Gene name	yxaL
Synonyms	yxaK
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yxaL
Interactions involving this protein in SubtInteract: YxaL
MW, pI	43 kDa, 6.379
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	yxaM, yxaJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification

This gene is a member of the following regulons

AbrB regulon, Rok regulon

The gene

Basic information

• Locus tag: BSU39940

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YxaL-PcrA PubMed

• Localization: extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• Structure:

• UniProt: P42111

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yxaJ-yxaL PubMed

• Expression browser: yxaL PubMed

• Sigma factor:

• Regulation:
  • repressed by Rok PubMed
  • repressed during logarithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • Rok: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • pGP392 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP824, available in Stülke lab

• 'lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: