MleA

• Description: malic enzyme
  
  

Gene name	mleA
Synonyms	yqkJ
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
Gene expression levels in SubtiExpress: mleA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	45 kDa, 4.895
Gene length, protein length	1317 bp, 439 aa
Immediate neighbours	yqkK, mleN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU23550

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate --> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): YtsJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54572

• KEGG entry: [2]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon:
  • ansA-ansB-mleN-mleA PubMed
  • mleN-mleA PubMed

• Expression browser: mleA PubMed

• Sigma factor:
  • ansA: SigA PubMed
  • mleN: SigA PubMed

• Regulation:
  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • mleN: repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1136 (cat) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed