DctS

• Description: two-component sensor kinase, regulation of the dctS-dctR-dctP operon
  
  

Gene name	dctS
Synonyms	ydbF
Essential	no
Product	two-component sensor kinase
Function	regulation of the dctS-dctR-dctP operon
Gene expression levels in SubtiExpress: dctS
Interactions involving this protein in SubtInteract: DctS
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	59 kDa, 6.198
Gene length, protein length	1605 bp, 535 aa
Immediate neighbours	dctB, dctR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU04450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of DctR

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DctS-DctR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96601

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dctS-dctR-dctP PubMed

• Expression browser: dctS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP225 (dctS::cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)