PbpG

• Description: penicillin-binding protein 2d
  
  

Gene name	pbpG
Synonyms	ywhE
Essential	no
Product	penicillin-binding protein 2d
Function	bifunctional glucosyltransferase/ transpeptidase
Gene expression levels in SubtiExpress: pbpG
MW, pI	71 kDa, 9.392
Gene length, protein length	1941 bp, 647 aa
Immediate neighbours	speE, ywhD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU37510

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • prespore Link to a summary

Database entries

• Structure:

• UniProt: P70997

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbpG (according to DBTBS)

• Expression browser: pbpG PubMed

• Sigma factor: SigF, SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF, SigG) PubMed

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between pbpG and ywhD PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

D C McPherson, A Driks, D L Popham
Two class A high-molecular-weight penicillin-binding proteins of Bacillus subtilis play redundant roles in sporulation.
J Bacteriol: 2001, 183(20);6046-53
[PubMed:11567005] [WorldCat.org] [DOI] (P p)

L B Pedersen, K Ragkousi, T J Cammett, E Melly, A Sekowska, E Schopick, T Murray, P Setlow
Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis.
Gene: 2000, 246(1-2);187-96
[PubMed:10767540] [WorldCat.org] [DOI] (P p)