Med

• Description: lipoprotein, positive regulator of comK, stimulates KinD-dependent phosphorylation of Spo0A
  
  

Gene name	med
Synonyms	yjaW
Essential	no
Product	positive regulator of comK
Function	regulation of competence
Gene expression levels in SubtiExpress: med
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	35 kDa, 4.599
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yjaV, comZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU11300

Phenotypes of a mutant

• impaired biofilm formation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates KinD-dependent phosphorylation of Spo0A PubMed

• Protein family: BMP lipoprotein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell surface PubMed

Database entries

• Structure:

• UniProt: O32436

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: med-comZ PubMed

• Expression browser: med PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additonal publications: PubMed

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hitoshi Hashimoto, Teruo Tanaka
Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis.
Biosci Biotechnol Biochem: 2002, 66(4);892-6
[PubMed:12036071] [WorldCat.org] [DOI] (P p)

M Ogura, Y Ohshiro, S Hirao, T Tanaka
A new Bacillus subtilis gene, med, encodes a positive regulator of comK.
J Bacteriol: 1997, 179(20);6244-53
[PubMed:9335269] [WorldCat.org] [DOI] (P p)