PhoA

• Description: alkaline phosphatase A
  
  

Gene name	phoA
Synonyms	phoAIV
Essential	no
Product	alkaline phosphatase A
Function	aquisition of phosphate upon phosphoate starvation
Gene expression levels in SubtiExpress: phoA
MW, pI	50 kDa, 9.926
Gene length, protein length	1383 bp, 461 aa
Immediate neighbours	spoVR, lytE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU09410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family: alkaline phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): PhoB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P19406

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon: phoA PubMed

• Expression browser: phoA PubMed

• Sigma factor: SigA PubMed, SigG PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A767 ( phoA::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage