RapI

• Description: response regulator aspartate phosphatase, antagonist of ImmR
  
  

Gene name	rapI
Synonyms	yddL
Essential	no
Product	response regulator aspartate phosphatase
Function	control of transfer of the mobile genetic element ICEBs1
Gene expression levels in SubtiExpress: rapI
Interactions involving this protein in SubtInteract: RapI
MW, pI	46 kDa, 5.054
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	yddK, phrI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, quorum sensing, mobile genetic elements

The gene

Basic information

• Locus tag: BSU05010

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds ImmR and inhibits its activity, this results in induction of the genes for the transfer of the mobile genetic element ICEBs1 PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrI indicates that neighbour cells do already contain the genetic element ICEBs1, this results in inactivation of RapI PubMed

• Interactions:
  • RapI-ImmR PubMed
  • RapI-PhrI PubMed

• Localization:

Database entries

• Structure: 4I1A PubMed

• UniProt: P96649

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapI-phrI PubMed

• Expression browser: rapI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Vijay Parashar, Philip D Jeffrey, Matthew B Neiditch
Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors.
PLoS Biol: 2013, 11(3);e1001512
[PubMed:23526881] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Katherine L Garrison, Alan D Grossman
Identification and characterization of the immunity repressor (ImmR) that controls the mobile genetic element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 64(6);1515-28
[PubMed:17511812] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)