rapI
168

response regulator aspartate phosphatase, antagonist of ImmR

Locus

BSU_05010

Molecular weight

46.40 kDa

Isoelectric point

5.05

Protein length

391 aaSequenceBlast

Gene length

1176 bpSequenceBlast

Function

control of transfer of the mobile genetic element ICEBs1

Product

response regulator aspartate phosphatase

Essential

Synonyms

rapI, yddL

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene

Coordinates

547,306 548,481

The protein

Catalyzed reaction/ biological activity

binds ImmR and inhibits its activity, this results in induction of the genes for the transfer of the mobile genetic element ICEBs1 PubMed

Protein family

RAP family (according to UniProt)

Domains

six tetratrichopeptide repeats (according to UniProt)

Structure

4I1A (PDB) PubMed

P96649 (AlphaFold)

Effectors of protein activity

binding of PhrI indicates that neighbour cells do already contain the genetic element ICEBs1, this results in inactivation of RapI PubMed

Expression and Regulation

Operons

Genes

rapI-phrI

Description

PubMed

Regulation

induced at high cell density (ComA) PubMed

Regulatory mechanism

ComA: activation, PubMed, in comA regulon

Sigma factors

SigH: sigma factor, PubMed, in sigH regulon

SigA: sigma factor, PubMed, in sigA regulon

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Biological materials

Mutant

BKE05010 (rapI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAAAATTCCCCCATCCT, downstream forward: _UP4_TTGATGAAAATCAGCCGGAT

BKK05010 (rapI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAAAATTCCCCCATCCT, downstream forward: _UP4_TTGATGAAAATCAGCCGGAT

References

Reviews

Original Publications

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Time of last update: 2025-04-02 20:05:58

Author of last update: Jstuelk

rapI 168

rapI
168