GerAC

• Description: lipoprotein, nutrient receptor, germination response to L-alanine
  
  

Gene name	gerAC
Synonyms
Essential	no
Product	nutrient receptor
Function	germination response to L-alanine
Gene expression levels in SubtiExpress: gerAC
Interactions involving this protein in SubtInteract: GerAC
MW, pI	42 kDa, 7.302
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	gerAB, liaR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU33070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gerABKC lipoprotein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerAA-GerAB-GerAC (tentative receptor complex) PubMed
  • GerAC is part of the germinosome in the spore inner membrane PubMed
  • GerAC-Lgt to fix GerAC to diacylglycerol in the membrane PubMed

• Localization: cell membrane (according to Swiss-Prot)
  • outer surface of the inner spore membrane PubMed

Database entries

• Structure:

• UniProt: P07870

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerAA-gerAB-gerAC PubMed

• Expression browser: gerAC PubMed

• Sigma factor: SigG PubMed, SigF PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed
  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

• Regulatory mechanism:
  • SpoVT: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Kerry-Ann V Stewart, Xuan Yi, Sonali Ghosh, Peter Setlow
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.
J Bacteriol: 2012, 194(12);3156-64
[PubMed:22493018] [WorldCat.org] [DOI] (I p)

Arturo Ramirez-Peralta, Pengfei Zhang, Yong-Qing Li, Peter Setlow
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Appl Environ Microbiol: 2012, 78(8);2689-97
[PubMed:22327596] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Wiyada Mongkolthanaruk, Gareth R Cooper, Julia S P Mawer, Raymond N Allan, Anne Moir
Effect of amino acid substitutions in the GerAA protein on the function of the alanine-responsive germinant receptor of Bacillus subtilis spores.
J Bacteriol: 2011, 193(9);2268-75
[PubMed:21378197] [WorldCat.org] [DOI] (I p)

Gareth R Cooper, Anne Moir
Amino acid residues in the GerAB protein important in the function and assembly of the alanine spore germination receptor of Bacillus subtilis 168.
J Bacteriol: 2011, 193(9);2261-7
[PubMed:21378181] [WorldCat.org] [DOI] (I p)

Venkata Ramana Vepachedu, Peter Setlow
Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores.
FEMS Microbiol Lett: 2007, 274(1);42-7
[PubMed:17573930] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Swaroopa Atluri, Katerina Ragkousi, Donna E Cortezzo, Peter Setlow
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor.
J Bacteriol: 2006, 188(1);28-36
[PubMed:16352818] [WorldCat.org] [DOI] (P p)

Takao Igarashi, Peter Setlow
Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores.
J Bacteriol: 2005, 187(7);2513-8
[PubMed:15774895] [WorldCat.org] [DOI] (P p)

Takao Igarashi, Barbara Setlow, Madan Paidhungat, Peter Setlow
Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis.
J Bacteriol: 2004, 186(10);2984-91
[PubMed:15126458] [WorldCat.org] [DOI] (P p)

Rosa-Martha Cabrera-Martinez, Federico Tovar-Rojo, Venkata Ramana Vepachedu, Peter Setlow
Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis.
J Bacteriol: 2003, 185(8);2457-64
[PubMed:12670969] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

I M Feavers, J Foulkes, B Setlow, D Sun, W Nicholson, P Setlow, A Moir
The regulation of transcription of the gerA spore germination operon of Bacillus subtilis.
Mol Microbiol: 1990, 4(2);275-82
[PubMed:2110996] [WorldCat.org] [DOI] (P p)

A R Zuberi, A Moir, I M Feavers
The nucleotide sequence and gene organization of the gerA spore germination operon of Bacillus subtilis 168.
Gene: 1987, 51(1);1-11
[PubMed:3110007] [WorldCat.org] [DOI] (P p)