CdaA

• Description: diadenylate cyclase, synthesis of c-di-AMP in vegetative cells
  
  

Gene name	cdaA
Synonyms	ybbP, ybbQ
Essential	no
Product	diadenylate cyclase
Function	synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaA
Interactions involving this protein in SubtInteract: CdaA
Metabolic function and regulation of this protein in SubtiPathways: Murein recycling
MW, pI	30 kDa, 8.074
Gene length, protein length	819 bp, 273 aa
Immediate neighbours	rsiW, cdaR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU01750

Phenotypes of a mutant

• inactivation of cdaA results in severe beta-lactam sensitivity PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • synthesis of c-di-AMP from two molecules of ATP PubMed

• Protein family:

• Paralogous protein(s): CdaS

Extended information on the protein

• Kinetic information:

• Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • the interaction with CdaR stimulates the diadenylate cyclase activity of CdaA PubMed

• Interactions:
  • CdaA-CdaR, to stimulate CdaA activity PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q45589

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cdaA-cdaR-glmM-glmS PubMed

• Expression browser: cdaA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • GP997 (cdaA::cat), available in Jörg Stülke's lab
  • GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab

• Expression vector:
  • expression of native cdaA in B. subtilis: pGP1960 (in pBQ200), available in Jörg Stülke's lab
  • expression of cdaA-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Jörg Stülke's lab

• lacZ fusion: GP1339 (cat) based on pAC6, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.

• FLAG-tag construct:
  • GP1331 lacA::cdaR-Strep aphA3 cdaA-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab
  • GP1381 cdaA-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

• Antibody:

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Additional publications: PubMed

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)