CdaS

• Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
  
  

Gene name	yojJ
Synonyms
Essential	no
Product	sporulation-specific diadenylate cyclase
Function	synthesis of c-di-AMP
Gene expression levels in SubtiExpress: yojJ
MW, pI	22 kDa, 5.367
Gene length, protein length	621 bp, 207 aa
Immediate neighbours	yojK, yojI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, sporulation proteins

The gene

Basic information

• Locus tag: BSU19430

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • synthesis of c-di-AMP from two molecules of ATP PubMed

• Protein family:

• Paralogous protein(s): CdaA

Extended information on the protein

• Kinetic information:

• Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)

• UniProt: O31854

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yojJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP983 (yojJ::ermC), available in Jörg Stülke's lab
  • GP1360 (yojJ::spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)