RocE

• Description: amino acid permease
  
  

Gene name	rocE
Synonyms
Essential	no
Product	amino acid permease
Function	arginine, ornithine and citrulline uptake
Gene expression levels in SubtiExpress: rocE
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	51 kDa, 8.528
Gene length, protein length	1401 bp, 467 aa
Immediate neighbours	rocF, rocD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU40330

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: secondary amino acid transporter

• Paralogous protein(s): AapA, GabP, HutM, RocC, YbgF, YbxG, YdgF, YtnA, YvbW

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• UniProt: P39137

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rocD-rocE-rocF PubMed

• Expression browser: rocE PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced by arginine (RocR, AhrC) PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • RocR, AhrC: transcription activation PubMed
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: