FruK

• Description: fructose-1-phosphate kinase
  
  

Gene name	fruK
Synonyms	fruB
Essential	no
Product	fructose-1-phosphate kinase
Function	fructose utilization
Gene expression levels in SubtiExpress: fruK
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 4.885
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	fruR, fruA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

FruR regulon

The gene

Basic information

• Locus tag: BSU14390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)

• Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3OHR

• UniProt: O31714

• KEGG entry: [3]

• E.C. number: 2.7.1.56

Expression and regulation

• Operon: fruR-fruK-fruA (according to DBTBS)

• Expression browser: fruK PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of fructose (FruR) (according to DBTBS)

• Regulatory mechanism:
  • FruR: transcription repression (according to DBTBS)
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)