GbsA

• Description: glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis
  
  

Gene name	gbsA
Synonyms
Essential	no
Product	glycine betaine-aldehyde dehydrogenase
Function	osmoprotection
Gene expression levels in SubtiExpress: gbsA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	53 kDa, 4.713
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	gbsB, gbsR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU31060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Betaine aldehyde + NAD⁺ + H₂O = betaine + NADH (according to Swiss-Prot)

• Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YfmT, AldY, YcbD, GabD, DhaS, IolA, PutC, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 31% identity, 67% similarity)

• UniProt: P71016

• KEGG entry: [2]

• E.C. number: 1.2.1.8

Expression and regulation

• Operon: gbsA-gbsB PubMed

• Expression browser: gbsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by choline (GbsR) PubMed

• Regulatory mechanism:
  • GbsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A1056 ( gbsA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

J Boch, G Nau-Wagner, S Kneip, E Bremer
Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
Arch Microbiol: 1997, 168(4);282-9
[PubMed:9297465] [WorldCat.org] [DOI] (P p)

J Boch, B Kempf, R Schmid, E Bremer
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
J Bacteriol: 1996, 178(17);5121-9
[PubMed:8752328] [WorldCat.org] [DOI] (P p)