SpsI

• Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis
  
  

Gene name	spsI
Synonyms	ipa-71d
Essential	no
Product	glucose-1-phosphate thymidylyltransferase
Function	rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsI
MW, pI	27 kDa, 5.385
Gene length, protein length	738 bp, 246 aa
Immediate neighbours	spsJ, spsG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU37840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed

• Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39629

• KEGG entry: [3]

• E.C. number: 2.7.7.24

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsI PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: