CwlD

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlD
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	spore germination
Gene expression levels in SubtiExpress: cwlD
MW, pI	26 kDa, 9.788
Gene length, protein length	711 bp, 237 aa
Immediate neighbours	ybaK, salA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

LexA regulon, SigE regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU01530

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • formation of muramic acid delta-lacton in peptidoglycan

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlC, LytC, YqiI, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P50864

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:
  • ybaK-cwlD PubMed
  • cwlD PubMed

• Expression browser: cwlD PubMed

• Sigma factor: SigE PubMed
  • ybaK: SigG PubMed
  • cwlD: SigE PubMed

• Regulation: expressed during sporulation (SigG, SigE) PubMed
  • repressed when no DNA damage is present (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed