SpoVG

• Description: negative effector of asymetric septation at the onset of sporulation
  
  

Gene name	spoVG
Synonyms
Essential	no
Product	negative effector of asymetric septation
Function	cell division, control of sporulation initiation
Gene expression levels in SubtiExpress: spoVG
MW, pI	10 kDa, 5.11
Gene length, protein length	291 bp, 97 aa
Immediate neighbours	yabJ, gcaD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell division, sporulation/ other, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, SigH regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU00490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: spoVG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2IA9

• UniProt: P28015

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: spoVG PubMed

• Expression browser: spoVG PubMed

• Sigma factor: SigH PubMed

• Regulation: repressed by SinR PubMed
  • repressed by SinR PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism: SinR: transcription repression PubMed
  • SinR: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hyun-Hwi Kim, Bong-Jin Lee, Ae-Ran Kwon
Expression, crystallization, and preliminary X-ray crystallographic analysis of putative SpoVG from Staphylococcus aureus.
Arch Pharm Res: 2010, 33(8);1285-8
[PubMed:20803133] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)

K Matsuno, A L Sonenshein
Role of SpoVG in asymmetric septation in Bacillus subtilis.
J Bacteriol: 1999, 181(11);3392-401
[PubMed:10348850] [WorldCat.org] [DOI] (P p)

O Resnekov, A Driks, R Losick
Identification and characterization of sporulation gene spoVS from Bacillus subtilis.
J Bacteriol: 1995, 177(19);5628-35
[PubMed:7559352] [WorldCat.org] [DOI] (P p)

P S Margolis, A Driks, R Losick
Sporulation gene spoIIB from Bacillus subtilis.
J Bacteriol: 1993, 175(2);528-40
[PubMed:8419299] [WorldCat.org] [DOI] (P p)

R Fürbass, M Gocht, P Zuber, M A Marahiel
Interaction of AbrB, a transcriptional regulator from Bacillus subtilis with the promoters of the transition state-activated genes tycA and spoVG.
Mol Gen Genet: 1991, 225(3);347-54
[PubMed:1850083] [WorldCat.org] [DOI] (P p)

P Zuber, R Losick
Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
J Bacteriol: 1987, 169(5);2223-30
[PubMed:2437099] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J Mol Biol: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

C D Banner, C P Moran, R Losick
Deletion analysis of a complex promoter for a developmentally regulated gene from Bacillus subtilis.
J Mol Biol: 1983, 168(2);351-65
[PubMed:6411929] [WorldCat.org] [DOI] (P p)

W C Johnson, C P Moran, R Losick
Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.
Nature: 1983, 302(5911);800-4
[PubMed:6405278] [WorldCat.org] [DOI] (P p)

J Segall, R Losick
Cloned Bacillus subtilis DNA containing a gene that is activated early during sporulation.
Cell: 1977, 11(4);751-61
[PubMed:408013] [WorldCat.org] [DOI] (P p)