MalS

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  • Description: malate dehydrogenase (decarboxylating), forms a transhydrogenation cycle with YtsJ for balancing of NADPH

Gene name malS
Synonyms
Essential no
Product malate dehydrogenase (decarboxylating)
Function malate utilization
Gene expression levels in SubtiExpress: malS
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 61 kDa, 4.895
Gene length, protein length 1698 bp, 566 aa
Immediate neighbours ytzB, ytnP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MalS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MalS expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1142 (spc) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References