CwlS

• Description: D,L-endopeptidase, peptidoglycan hydrolase
  
  

Gene name	cwlS
Synonyms	yojL
Essential	no
Product	D,L-endopeptidase, peptidoglycan hydrolase
Function	cell wall metabolism
Gene expression levels in SubtiExpress: cwlS
MW, pI	44 kDa, 10.438
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	yojM, yojK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

Abh regulon, AbrB regulon, CcpA regulon, SigD regulon, SigH regulon,

The gene

Basic information

• Locus tag: BSU19410

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A₂pm) in 7-(L-Ala-gamma-D-Glu)-meso-A₂pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A₂pm (according to Swiss-Prot)

• Protein family: Cu-Zn superoxide dismutase family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• Structure:

• UniProt: O31852

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cwlS PubMed

• Sigma factor: SigD, SigH, according to PubMed

• Regulation:
  • repressed by glucose (3-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • Abh and AbrB: synergistic transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Masayuki Hashimoto, Seika Ooiwa, Junichi Sekiguchi
Synthetic lethality of the lytE cwlO genotype in Bacillus subtilis is caused by lack of D,L-endopeptidase activity at the lateral cell wall.
J Bacteriol: 2012, 194(4);796-803
[PubMed:22139507] [WorldCat.org] [DOI] (I p)

Tatsuya Fukushima, Anahita Afkham, Shin-Ichirou Kurosawa, Taichi Tanabe, Hiroki Yamamoto, Junichi Sekiguchi
A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis.
J Bacteriol: 2006, 188(15);5541-50
[PubMed:16855244] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)