CtsR

Gene name	ctsR
Synonyms	yacG
Essential	no
Product	transcription repressor
Function	regulation of protein degradation
Gene expression levels in SubtiExpress: ctsR
Interactions involving this protein in SubtInteract: CtsR
Regulatory function and regulation of this protein in SubtiPathways: Stress, Phosphorelay
MW, pI	17 kDa, 9.261
Gene length, protein length	462 bp, 154 aa
Immediate neighbours	rrnW-5S, mcsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, transcription factors and their control, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU00830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ctsR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylation of a tyrosine residue by McsB PubMed
  • recently, it was reported that CtsR is phosphorylated by McsB on Arg-62 rather than on a tyrosine residue PubMed
  • in addition, Arg-15 was reported to be a phosphorylation site PubMed

• Cofactor(s):

• Effectors of protein activity:
  • CtsR is inactivated by heat, heat sensing requires Gly-64 PubMed
  • non-phosphorylated McsB targets CtsR for degradation PubMed
  • regulated proteolysis by ClpP/ClpC PubMed

• Interactions:
  • McsA-McsB-CtsR
  • CtsR-ClpP/ClpC (degradation of CtsR) PubMed

• Localization:

Database entries

• Structure: 3H0D (complex with a 26bp DNA duplex, from Geobacillus stearothermophilus) PubMed

• UniProt: P37568

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: ctsR PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2

• Regulation:
  • induced by stress (SigB) PubMed
  • repressed at low temperatures (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant: ctsR::aphA3 availbale from the Gerth lab

ctsRG65P::spec available from the Gerth lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab

References

Reviews

Alexander K W Elsholz, Ulf Gerth, Michael Hecker
Regulation of CtsR activity in low GC, Gram+ bacteria.
Adv Microb Physiol: 2010, 57;119-44
[PubMed:21078442] [WorldCat.org] [DOI] (I p)

Wolfgang Schumann
The Bacillus subtilis heat shock stimulon.
Cell Stress Chaperones: 2003, 8(3);207-17
[PubMed:14984053] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed