ExoA

• Description: apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance
  
  

Gene name	exoA
Synonyms
Essential	no
Product	apurinic/apyrimidinic endonuclease
Function	repair of oxidative DNA damage in spores
MW, pI	29 kDa, 4.592
Gene length, protein length	756 bp, 252 aa
Immediate neighbours	ccpB, rpsR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU40880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• ExoA is functionally redundant with Nfo

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)

• Protein family: DNA repair enzymes AP/exoA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37454

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: exoA-ccpB (according to DBTBS)

• Expression browser: exoA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • available in Mario Pedraza-Reyes' lab
  • GP898 (exoA::kan), available in Stülke lab
  • GP1503 (exoA::kan, nfo::cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Marcelo Barraza-Salas, Juan R Ibarra-Rodríguez, Silvia J Mellado, José M Salas-Pacheco, Peter Setlow, Mario Pedraza-Reyes
Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores.
FEMS Microbiol Lett: 2010, 302(2);159-65
[PubMed:19930460] [WorldCat.org] [DOI] (I p)

Juan R Ibarra, Alma D Orozco, Juan A Rojas, Karina López, Peter Setlow, Ronald E Yasbin, Mario Pedraza-Reyes
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores.
J Bacteriol: 2008, 190(6);2031-8
[PubMed:18203828] [WorldCat.org] [DOI] (I p)

José M Salas-Pacheco, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.
J Bacteriol: 2005, 187(21);7374-81
[PubMed:16237020] [WorldCat.org] [DOI] (P p)

T Shida, T Ogawa, N Ogasawara, J Sekiguchi
Characterization of Bacillus subtilis ExoA protein: a multifunctional DNA-repair enzyme similar to the Escherichia coli exonuclease III.
Biosci Biotechnol Biochem: 1999, 63(9);1528-34
[PubMed:10540738] [WorldCat.org] [DOI] (P p)