SunS

• Description: sublancin S-glycosyltransferase
  
  

Gene name	yolJ
Synonyms
Essential	no
Product	sublancin S-glycosyltransferase
Function	biosynthesis of the antimicrobial peptide sublancin
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	49 kDa, 5.33
Gene length, protein length	1266 bp, 422 aa
Immediate neighbours	bdbB, bdbA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

SP-beta prophage, membrane proteins, protein modification, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

Abh regulon, AbrB regulon, Rok regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU21450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • selectively modifies Cys22 in a 56-amino acid peptide substrate SunA and can accept a variety of NDP-sugars PubMed

• Protein family: EngA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31986

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sunT-bdbA-sunS-bdbB PubMed

• Sigma factor:

• Regulation:
  • repressed by Rok PubMed

• Regulatory mechanism:
  • activated by YvrHb PubMed
  • Rok: transcription repression PubMed
  • AbrB: transcription repressionPubMed
  • Abh: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Trent J Oman, John M Boettcher, Huan Wang, Xenia N Okalibe, Wilfred A van der Donk
Sublancin is not a lantibiotic but an S-linked glycopeptide.
Nat Chem Biol: 2011, 7(2);78-80
[PubMed:21196935] [WorldCat.org] [DOI] (I p)

Mark Albano, Wiep Klaas Smits, Linh T Y Ho, Barbara Kraigher, Ines Mandic-Mulec, Oscar P Kuipers, David Dubnau
The Rok protein of Bacillus subtilis represses genes for cell surface and extracellular functions.
J Bacteriol: 2005, 187(6);2010-9
[PubMed:15743949] [WorldCat.org] [DOI] (P p)