MecA
- Description: buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A∼P by direct interaction
| Gene name | mecA |
| Synonyms | |
| Essential | no |
| Product | adaptor protein |
| Function | control of ComK degradation, regulation of competence |
| MW, pI | 25 kDa, 4.209 |
| Gene length, protein length | 654 bp, 218 aa |
| Immediate neighbours | yjbE, coiA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
genetic competence, proteolysis
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11520
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: mecA family (according to Swiss-Prot)
- Paralogous protein(s): YpbH
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- UniProt: P37958
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews: PubMed
Original Publications
Additonal publications: PubMed
Douglas J Kojetin, Patrick D McLaughlin, Richele J Thompson, David Dubnau, Peter Prepiak, Mark Rance, John Cavanagh
Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions.
J Mol Biol: 2009, 387(3);639-52
[PubMed:19361434]
[WorldCat.org]
[DOI]
(I p)
Peter Prepiak, David Dubnau
A peptide signal for adapter protein-mediated degradation by the AAA+ protease ClpCP.
Mol Cell: 2007, 26(5);639-47
[PubMed:17560370]
[WorldCat.org]
[DOI]
(P p)
Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol Microbiol: 2002, 43(5);1331-45
[PubMed:11918817]
[WorldCat.org]
[DOI]
(P p)
Marjan Persuh, Ines Mandic-Mulec, David Dubnau
A MecA paralog, YpbH, binds ClpC, affecting both competence and sporulation.
J Bacteriol: 2002, 184(8);2310-3
[PubMed:11914365]
[WorldCat.org]
[DOI]
(P p)
K Turgay, J Hahn, J Burghoorn, D Dubnau
Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor.
EMBO J: 1998, 17(22);6730-8
[PubMed:9890793]
[WorldCat.org]
[DOI]
(P p)
K Turgay, L W Hamoen, G Venema, D Dubnau
Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis.
Genes Dev: 1997, 11(1);119-28
[PubMed:9000055]
[WorldCat.org]
[DOI]
(P p)
J Hahn, J Bylund, M Haines, M Higgins, D Dubnau
Inactivation of mecA prevents recovery from the competent state and interferes with cell division and the partitioning of nucleoids in Bacillus subtilis.
Mol Microbiol: 1995, 18(4);755-67
[PubMed:8817496]
[WorldCat.org]
[DOI]
(P p)
L Kong, K J Siranosian, A D Grossman, D Dubnau
Sequence and properties of mecA, a negative regulator of genetic competence in Bacillus subtilis.
Mol Microbiol: 1993, 9(2);365-73
[PubMed:8412687]
[WorldCat.org]
[DOI]
(P p)
M Roggiani, J Hahn, D Dubnau
Suppression of early competence mutations in Bacillus subtilis by mec mutations.
J Bacteriol: 1990, 172(7);4056-63
[PubMed:2113920]
[WorldCat.org]
[DOI]
(P p)
