RocR

• Description: transcriptional activator of arginine utilization operons
  
  

Gene name	rocR
Synonyms
Essential	no
Product	transcriptional regulator
Function	transcriptional activator of arginine utilization operons
MW, pI	52.6 kDa, 6.08
Gene length, protein length	1383 bp, 461 amino acids
Immediate neighbours	rocD, yyxA
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates: 4144771 - 4146153

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG

• Protein family: Sigma-54 interacting transcription activator

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • Sigma-54 factor interaction domain (143–372)
  • 2x nucleotid-binding domain (171–178),(233–242)
  • HTH motif (434–453)

• Modification:

• Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed

• Effectors of protein activity:

• Interactions: RocR-SigL

• Localization:

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

Expression and regulation

• Operon: rocR

• Sigma factor: SigA PubMed

• Regulation: autoregulation by RocR PubMed

• Regulatory mechanism: autorepression

• Additional information:

Biological materials

• Mutant: QB5533 (aphA3), from Michel Debarbouille, available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

1. Belitsky BR, Sonenshein AL (1998) Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J Bacteriol 180:6298-6305. PubMed
2. Belitsky BR, Sonenshein, AL: (1999) An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis. Proc Natl Acad Sci USA , 96:10290-10295. PubMed
3. Gardan R, Rapoport G, Débarbouillé M:(1997) Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis. Mol Microbiol , 24:825-837. PubMed
4. Gardan R, Rapoport G, Débarbouillé M: (1995) Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol , 249:843-856. PubMed
5. Ali, N. O., J. Jeusset, E. Larquet, E. le Cam, B. Belitsky, A. L. Sonenshein, T. Msadek, and M. Débarbouillé. 2003. Specificity of the interaction of RocR with the rocG-rocA intergenic region in Bacillus subtilis. Microbiology 149: 739-750. PubMed