CtsR

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  • Description: transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)

Gene name ctsR
Synonyms yacG
Essential no
Product transcription repressor
Function regulation of protein degradation
Regulatory function and regulation of this protein in SubtiPathways:
Stress, Phosphorelay
MW, pI 17 kDa, 9.261
Gene length, protein length 462 bp, 154 aa
Immediate neighbours rrnW-5S, mcsA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CtsR context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU00830

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

proteolysis, transcription factors and their control, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ctsR family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylation of a tyrosine residue by McsB PubMed
    • recently, it was reported that CtsR is phosphorylated by McsB on Arg-62 rather than on a tyrosine residue PubMed
  • Cofactor(s):
  • Effectors of protein activity:
    • CtsR is inactivated by heat, heat sensing requires Gly-64 PubMed
    • non-phosphorylated McsB targets CtsR for degradation PubMed
    • regulated proteolysis by ClpP/ClpC PubMed
  • Localization:

Database entries

  • Structure: 3H0D (complex with a 26bp DNA duplex, from Geobacillus stearothermophilus) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Alexander K W Elsholz, Ulf Gerth, Michael Hecker
Regulation of CtsR activity in low GC, Gram+ bacteria.
Adv Microb Physiol: 2010, 57;119-44
[PubMed:21078442] [WorldCat.org] [DOI] (I p)

Wolfgang Schumann
The Bacillus subtilis heat shock stimulon.
Cell Stress Chaperones: 2003, 8(3);207-17
[PubMed:14984053] [WorldCat.org] [DOI] (P p)


Original publications

Additional publications: PubMed