LiaS

• Description: two-component sensor kinase, response to bacitracin
  
  

Gene name	liaS
Synonyms	yvqE
Essential	no
Product	two-component sensor kinase
Function	control of LiaR activity in response to bacitracin
MW, pI	40 kDa, 7.333
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	liaR, liaF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of LiaR

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LiaS-LiaR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32198

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • liaI-liaH-liaG-liaF-liaS-liaR PubMed
  • liaG-liaF-liaS-liaR PubMed

• Sigma factor:
  • liaI: SigA PubMed
  • liaG: SigA PubMed

• Regulation:
  • liaI: induced by bacitracin (LiaR) PubMed
  • liaI:repressed during logarithmic growth (AbrB) PubMed
  • liaG: constitutive

• Regulatory mechanism:
  • LiaR: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage