Dat
- Description: D-alanine aminotransferase
(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt)
| Gene name | dat |
| Synonyms | yheM |
| Essential | no |
| Product | D-alanine aminotransferase |
| Function | peptidoglycan precursor biosynthesis |
| Metabolic function and regulation of this protein in SubtiPathways: Cell wall | |
| MW, pI | 31 kDa, 4.871 |
| Gene length, protein length | 846 bp, 282 aa |
| Immediate neighbours | yheN, nhaC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU09670
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)
- Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)
- UniProt: O07597
- KEGG entry: [2]
- E.C. number: 2.6.1.21
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219]
[WorldCat.org]
[DOI]
(P p)
P P Taylor, I G Fotheringham
Nucleotide sequence of the Bacillus licheniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species.
Biochim Biophys Acta: 1997, 1350(1);38-40
[PubMed:9003455]
[WorldCat.org]
[DOI]
(P p)
