BacD

• Description: alanine-anticapsin ligase
  
  

Gene name	bacD
Synonyms	ywfE, ipa-83d
Essential	no
Product	alanine-anticapsin ligase
Function	biosynthesis of the antibiotic bacilysin
MW, pI	52 kDa, 4.679
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	bacE, bacC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37710

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + an L-amino acid + an L-amino acid = ADP + phosphate + L-aminoacyl-L-amino acid (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39641

• KEGG entry: [2]

• E.C. number: 6.3.2.28

Expression and regulation

• Operon: bacA-bacB-bacC-bacD-bacE-bacF PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Kuniki Kino, Yoichi Kotanaka, Toshinobu Arai, Makoto Yagasaki
A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin.
Biosci Biotechnol Biochem: 2009, 73(4);901-7
[PubMed:19352016] [WorldCat.org] [DOI] (I p)

Kazuhiko Tabata, Hajime Ikeda, Shin-Ichi Hashimoto
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
J Bacteriol: 2005, 187(15);5195-202
[PubMed:16030213] [WorldCat.org] [DOI] (P p)

Gerhard Steinborn, Mohammad-Reza Hajirezaei, Jürgen Hofemeister
bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains.
Arch Microbiol: 2005, 183(2);71-9
[PubMed:15609023] [WorldCat.org] [DOI] (P p)

Ayten Yazgan Karataş, Seçil Cetin, Gülay Ozcengiz
The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis.
Biochim Biophys Acta: 2003, 1626(1-3);51-6
[PubMed:12697329] [WorldCat.org] (P p)

Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825] [WorldCat.org] [DOI] (P p)