MtnK

• Description: 5-methylthioribose kinase
  
  

Gene name	mtnK
Synonyms	ykrT
Essential	no
Product	5-methylthioribose kinase
Function	methionine salvage
MW, pI	45 kDa, 4.856
Gene length, protein length	1197 bp, 399 aa
Immediate neighbours	mtnS, mtnU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family: methylthioribose kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane associated PubMed

Database entries

• Structure: 2PUP

• Swiss prot entry: O31663

• KEGG entry: [3]

• E.C. number: 2.7.1.100

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: mtnK-mtnS PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
2. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed