mtnK

mtnK
168

5-methylthioribose kinase

Locus
BSU_13560
Molecular weight
45.19 kDa
Isoelectric point
4.86
Protein length
399 aaSequenceBlast
Gene length
1200 bpSequenceBlast
Function
methionine salvage
Product
5-methylthioribose kinase
Essential
no
E.C.
2.7.1.100
Synonyms
mtnK, ykrT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4857 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,423,241 1,424,440
The protein
Catalyzed reaction/ biological activity
ATP + S-methyl-5-thio-D-ribose --> ADP + H+ + S-methyl-5-thio--D-ribose 1-phosphate (according to UniProt)
Protein family
methylthioribose kinase family (single member, according to UniProt)
Structure
2PUP (PDB)
O31663 (AlphaFold)
Modification
phosphorylated on Arg-80 OR Arg-82 and Arg-365 PubMed
Localization
membrane associated PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
mtnK-mtnA
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
NusG-dependent transcription pausing precedes the completion of the SAM-binding aptamer domain of the S-box riboswitch PubMed
the S-box RNA is degraded by RNase Y PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

mtnK→mtnA

2025-03-28 05:34:22

Jstuelk

99

5E437FB23A4F009B0E9A667E3F04D466554C0F58

2EB65B4B0A58D80719B6A9288A8B2BEF4BC3FFF2

Biological materials
Mutant
MGNA-A783 (ykrT::erm), available at the NBRP B. subtilis, Japan
BKE13560 (mtnK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACCTCCAATTATGTAA, downstream forward: _UP4_TGATCTATTCATGACCCATT
BKK13560 (mtnK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACCTCCAATTATGTAA, downstream forward: _UP4_TGATCTATTCATGACCCATT
References
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E2475C56019C8EA3E05DC8FF89D8FC2751FA2544

Page visits: 4588

Time of last update: 2025-03-31 08:00:28

Author of last update: Jstuelk