Sandbox

• Description: ATP-dependent Clp protease-like (class III stress gene)
  
  

Gene name	clpE
Synonyms	ykvH
Essential	no
Product	ATP-dependent Clp protease-like
Function	protein degradation
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	77 kDa, 5.135
Gene length, protein length	2097 bp, 699 aa
Immediate neighbours	motA, ykvI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATPase/chaperone

• Protein family: AAA+ -type ATPase (IPR013093) InterPro (PF07724) PFAM

• Paralogous protein(s): ClpC, ClpX

Extended information on the protein

• Kinetic information:

• Domains: AAA-ATPase PFAM

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ClpE-ClpP

• Localization: expressed after heat shock, localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed

Database entries

• Structure:

• Swiss prot entry: O31673

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: induced by heat (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed1, PubMed2, PubMed3, PubMed4

• Additional information:

Biological materials

• Mutant: clpE::spec available from the Hamoen] Lab

• Expression vector:

• lacZ fusion:

• GFP fusion: C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Leendert Hamoen, Newcastle University, UK homepage

References

1. Kirstein, J., Strahl, H., Molière, N., Hamoen, LW., Turgay K. (2008) Localization of general and regulatory proteolysis in Bacillus subtilis cells. Mol Microbiol. 70:682-94. Pubmed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed