YtsJ

• Description: malic enzyme
  
  

Gene name	ytsJ
Synonyms
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
MW, pI	43 kDa, 5.046
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	accD, dnaE
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: malate <--> pyruvate

• Protein family:

• Paralogous protein(s): MleA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number: 1.1.1.40

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP612 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
2. Lerondel G, Doan T, Zamboni N, Sauer U, Aymerich S. (2006) YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis. J Bacteriol. 188: 4727-4736. PubMed
3. Doan, T., P. Servant, S. Tojo, H. Yamaguchi, G. Lerondel, K. Yoshida, Y. Fujita, and S. Aymerich. 2003. The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate. Microbiology 149: 2331-2343. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed