ProJ

• Description: glutamate 5-kinase
  
  

Gene name	proJ
Synonyms	yohA
Essential	no
Product	glutamate 5-kinase
Function	osmoadaptive de novo production of proline
Gene expression levels in SubtiExpress: proJ
Metabolic function and regulation of this protein in SubtiPathways: proJ
MW, pI	40 kDa, 5.296
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	gltC, proH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18470

Database entries

• BsubCyc: BSU18470

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): ProB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU18470

• Structure:

• UniProt: O07509

• KEGG entry: [2]

• E.C. number: 2.7.2.11

Expression and regulation

• Operon: proH-proJ PubMed

• Expression browser: proJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP817 (spc) available in the Stülke lab
  • 1A635 ( proJ::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed