proJ

proJ
168

glutamate 5-kinase

Locus
BSU_18470
Molecular weight
40.20 kDa
Isoelectric point
5.3
Protein length
371 aaSequenceBlast
Gene length
1116 bpSequenceBlast
Function
osmoadaptive de novo production of proline
Product
glutamate 5-kinase
Essential
no
E.C.
2.7.2.11
Synonyms
proJ, yohA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,015,733  2,016,848
The protein
Catalyzed reaction/ biological activity
ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)
Protein family
glutamate 5-kinase family (with ProB, according to UniProt)
Domains
PUA domain (aa 280-356) (according to UniProt)
Structure
4Q1T (PDB) (from Burkholderia thailandensis, 39% identity)
O07509 (AlphaFold)
Paralogous protein(s)
ProB
Expression and Regulation
Operons
Genes
proH-proJ
Description
PubMed
Regulation
expressed under conditions of osmotic stress PubMed
induced upon antibiotic stress PubMed
Expression
expression is heterogeneously activated PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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proH→proJ

2025-03-16 09:00:03

Jstuelk

85

7D11E4548AED5470042D4AD4B1A81BAF2078CCD0

AED34EA4995FE0B1F5AA7A879B3AA26AFD68CDB8

Biological materials
Mutant
GP817 (spc) available in Jörg Stülke's lab
1A635 ( proJ::erm), PubMed, available at BGSC
BKE18470 (proJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG,  downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
BKK18470 (proJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG,  downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
References
Reviews
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Original Publications
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3F47513FB71276EF1E99B8C427DC8DC0786F8C19

Page visits: 2400

Time of last update: 2025-04-02 02:11:52

Author of last update: Jstuelk