SunS

• Description: sublancin S-glycosyltransferase
  
  

Gene name	yolJ
Synonyms
Essential	no
Product	sublancin S-glycosyltransferase
Function	biosynthesis of the antimicrobial peptide sublancin
Gene expression levels in SubtiExpress: sunS
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	49 kDa, 5.33
Gene length, protein length	1266 bp, 422 aa
Immediate neighbours	bdbB, bdbA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

SP-beta prophage, membrane proteins, protein modification, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

Abh regulon, AbrB regulon, Rok regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU21450

Database entries

• BsubCyc: BSU21450

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • selectively modifies Cys22 in a 56-amino acid peptide substrate SunA and can accept a variety of NDP-sugars PubMed

• Protein family: EngA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU21450

• Structure:

• UniProt: O31986

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sunT-bdbA-sunS-bdbB PubMed

• Expression browser: sunS PubMed

• Sigma factor:

• Regulation:
  • repressed by Rok PubMed

• Regulatory mechanism:
  • activated by YvrHb PubMed
  • Rok: transcription repression PubMed
  • AbrB: transcription repressionPubMed
  • Abh: transcription activation PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Huan Wang, Wilfred A van der Donk
Substrate selectivity of the sublancin S-glycosyltransferase.
J Am Chem Soc: 2011, 133(41);16394-7
[PubMed:21910430] [WorldCat.org] [DOI] (I p)

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)

Trent J Oman, John M Boettcher, Huan Wang, Xenia N Okalibe, Wilfred A van der Donk
Sublancin is not a lantibiotic but an S-linked glycopeptide.
Nat Chem Biol: 2011, 7(2);78-80
[PubMed:21196935] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Mark Albano, Wiep Klaas Smits, Linh T Y Ho, Barbara Kraigher, Ines Mandic-Mulec, Oscar P Kuipers, David Dubnau
The Rok protein of Bacillus subtilis represses genes for cell surface and extracellular functions.
J Bacteriol: 2005, 187(6);2010-9
[PubMed:15743949] [WorldCat.org] [DOI] (P p)