SpoIIIE

• Description: ATP-dependent DNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
  
  

Gene name	spoIIIE
Synonyms
Essential	no
Product	ATP-dependent DNA translocase required for chromosome partitioning through the septum into the forespore
Function	chromosome partition during sporulation
Gene expression levels in SubtiExpress: spoIIIE
MW, pI	86 kDa, 6.757
Gene length, protein length	2361 bp, 787 aa
Immediate neighbours	ylzJ, ymfC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU16800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • transports the forespore chromosome across the sporulation septum PubMed
  • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed
  • binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG PubMed

• Protein family:

• Paralogous protein(s): SftA

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal transmembrane domain (4 helices) PubMed
  • 134 aa linker PubMed
  • 512 aa motor domain at the C-terminus PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • assembles into ∼45 nm complexes PubMed

• Localization:
  • transmembrane domain mediates dynamic localization to active division sites PubMed
  • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

Database entries

• Structure:

• UniProt: P21458

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIE PubMed

• Expression browser: spoIIIE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Xindan Wang, Paula Montero Llopis, David Z Rudner
Organization and segregation of bacterial chromosomes.
Nat Rev Genet: 2013, 14(3);191-203
[PubMed:23400100] [WorldCat.org] [DOI] (I p)

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Christine Kaimer, Peter L Graumann
Players between the worlds: multifunctional DNA translocases.
Curr Opin Microbiol: 2011, 14(6);719-25
[PubMed:22047950] [WorldCat.org] [DOI] (I p)

Original publications

Diego I Cattoni, Shreyasi Thakur, Cedric Godefroy, Antoine Le Gall, Josephine Lai-Kee-Him, Pierre-Emmanuel Milhiet, Patrick Bron, Marcelo Nöllmann
Structure and DNA-binding properties of the Bacillus subtilis SpoIIIE DNA translocase revealed by single-molecule and electron microscopies.
Nucleic Acids Res: 2014, 42(4);2624-36
[PubMed:24297254] [WorldCat.org] [DOI] (I p)

Jean-Bernard Fiche, Diego I Cattoni, Nele Diekmann, Julio Mateos Langerak, Caroline Clerte, Catherine A Royer, Emmanuel Margeat, Thierry Doan, Marcelo Nöllmann
Recruitment, assembly, and molecular architecture of the SpoIIIE DNA pump revealed by superresolution microscopy.
PLoS Biol: 2013, 11(5);e1001557
[PubMed:23667326] [WorldCat.org] [DOI] (I p)

Diego I Cattoni, Osvaldo Chara, Cédric Godefroy, Emmanuel Margeat, Sonia Trigueros, Pierre-Emmanuel Milhiet, Marcelo Nöllmann
SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation.
EMBO Rep: 2013, 14(5);473-9
[PubMed:23559069] [WorldCat.org] [DOI] (I p)

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Tinya C Fleming, Jae Yen Shin, Sang-Hyuk Lee, Eric Becker, Kerwyn Casey Huang, Carlos Bustamante, Kit Pogliano
Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus subtilis sporulation.
Genes Dev: 2010, 24(11);1160-72
[PubMed:20516200] [WorldCat.org] [DOI] (I p)

David J Sherratt, Lidia K Arciszewska, Estelle Crozat, James E Graham, Ian Grainge
The Escherichia coli DNA translocase FtsK.
Biochem Soc Trans: 2010, 38(2);395-8
[PubMed:20298190] [WorldCat.org] [DOI] (I p)

Christine Kaimer, José Eduardo González-Pastor, Peter L Graumann
SpoIIIE and a novel type of DNA translocase, SftA, couple chromosome segregation with cell division in Bacillus subtilis.
Mol Microbiol: 2009, 74(4);810-25
[PubMed:19818024] [WorldCat.org] [DOI] (I p)

Steven J Biller, William F Burkholder
The Bacillus subtilis SftA (YtpS) and SpoIIIE DNA translocases play distinct roles in growing cells to ensure faithful chromosome partitioning.
Mol Microbiol: 2009, 74(4);790-809
[PubMed:19788545] [WorldCat.org] [DOI] (I p)

Kathleen A Marquis, Briana M Burton, Marcelo Nollmann, Jerod L Ptacin, Carlos Bustamante, Sigal Ben-Yehuda, David Z Rudner
SpoIIIE strips proteins off the DNA during chromosome translocation.
Genes Dev: 2008, 22(13);1786-95
[PubMed:18593879] [WorldCat.org] [DOI] (P p)

Jerod L Ptacin, Marcelo Nollmann, Eric C Becker, Nicholas R Cozzarelli, Kit Pogliano, Carlos Bustamante
Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis.
Nat Struct Mol Biol: 2008, 15(5);485-93
[PubMed:18391964] [WorldCat.org] [DOI] (I p)

Briana M Burton, Kathleen A Marquis, Nora L Sullivan, Tom A Rapoport, David Z Rudner
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell: 2007, 131(7);1301-12
[PubMed:18160039] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
Assembly of the SpoIIIE DNA translocase depends on chromosome trapping in Bacillus subtilis.
Curr Biol: 2003, 13(24);2196-200
[PubMed:14680637] [WorldCat.org] (P p)

K P Lemon, I Kurtser, A D Grossman
Effects of replication termination mutants on chromosome partitioning in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2001, 98(1);212-7
[PubMed:11134515] [WorldCat.org] [DOI] (P p)

J Bath, L J Wu, J Errington, J C Wang
Role of Bacillus subtilis SpoIIIE in DNA transport across the mother cell-prespore division septum.
Science: 2000, 290(5493);995-7
[PubMed:11062134] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Use of asymmetric cell division and spoIIIE mutants to probe chromosome orientation and organization in Bacillus subtilis.
Mol Microbiol: 1998, 27(4);777-86
[PubMed:9515703] [WorldCat.org] [DOI] (P p)

K Pogliano, A E Hofmeister, R Losick
Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1997, 179(10);3331-41
[PubMed:9150232] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Septal localization of the SpoIIIE chromosome partitioning protein in Bacillus subtilis.
EMBO J: 1997, 16(8);2161-9
[PubMed:9155041] [WorldCat.org] [DOI] (P p)

M E Sharpe, J Errington
Postseptational chromosome partitioning in bacteria.
Proc Natl Acad Sci U S A: 1995, 92(19);8630-4
[PubMed:7567988] [WorldCat.org] [DOI] (P p)

L J Wu, P J Lewis, R Allmansberger, P M Hauser, J Errington
A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.
Genes Dev: 1995, 9(11);1316-26
[PubMed:7797072] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division.
Science: 1994, 264(5158);572-5
[PubMed:8160014] [WorldCat.org] [DOI] (P p)

D Foulger, J Errington
The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1247-55
[PubMed:2507870] [WorldCat.org] [DOI] (P p)

S Panzer, R Losick, D Sun, P Setlow
Evidence for an additional temporal class of gene expression in the forespore compartment of sporulating Bacillus subtilis.
J Bacteriol: 1989, 171(1);561-4
[PubMed:2492502] [WorldCat.org] [DOI] (P p)

P D Butler, J Mandelstam
Nucleotide sequence of the sporulation operon, spoIIIE, of Bacillus subtilis.
J Gen Microbiol: 1987, 133(9);2359-70
[PubMed:3129532] [WorldCat.org] [DOI] (P p)