CwlD

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlD
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	spore germination
Gene expression levels in SubtiExpress: cwlD
MW, pI	26 kDa, 9.788
Gene length, protein length	711 bp, 237 aa
Immediate neighbours	ybaK, salA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

LexA regulon, SigE regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU01530

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • formation of muramic acid delta-lacton in peptidoglycan

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlC, LytC, YqiI, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P50864

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:
  • ybaK-cwlD PubMed
  • cwlD PubMed

• Expression browser: cwlD PubMed

• Sigma factor: SigE PubMed
  • ybaK: SigG PubMed
  • cwlD: SigE PubMed

• Regulation: expressed during sporulation (SigG, SigE) PubMed
  • repressed when no DNA damage is present (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: