FruK

• Description: fructose-1-phosphate kinase
  
  

Gene name	fruK
Synonyms	fruB
Essential	no
Product	fructose-1-phosphate kinase
Function	fructose utilization
Gene expression levels in SubtiExpress: fruK
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 4.885
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	fruR, fruA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

FruR regulon

The gene

Basic information

• Locus tag: BSU14390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)

• Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3OHR

• UniProt: O31714

• KEGG entry: [3]

• E.C. number: 2.7.1.56

Expression and regulation

• Operon: fruR-fruK-fruA (according to DBTBS)

• Expression browser: fruK PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of fructose (FruR) (according to DBTBS)

• Regulatory mechanism:
  • FruR: transcription repression (according to DBTBS)
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)